Recombinant Human PDE1B protein, His&GST-tagged

• Cat.No.: PDE1B-1164H
• Product Overview: Recombinant Human PDE1B protein(Q01064-1) (Met 1-Asp 536) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-536 aa
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0.
• Molecular Mass: The recombinant human PDE1B/GST chimera consists of 773 amino acids and has a calculated molecular mass of 89.2 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 94 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PDE1B phosphodiesterase 1B, calmodulin-dependent [ Homo sapiens ]
• Official Symbol: PDE1B
• Synonyms: PDE1B; phosphodiesterase 1B, calmodulin-dependent; PDES1B; calcium/calmodulin-dependent 3,5-cyclic nucleotide phosphodiesterase 1B; cam-PDE 1B; 63 kDa Cam-PDE; calmodulin-stimulated phosphodiesterase PDE1B1; calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase; presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family known as PDE1B; PDE1B1
• Gene ID: 5153
• mRNA Refseq: NM_000924
• Protein Refseq: NP_000915
• MIM: 171891
• UniProt ID: Q01064