Active Recombinant Human PDGFB Protein, Biotinylated
Cat.No. : | PDGFB-168H |
Product Overview : | Recombinant Human PDGFB(Ser82-Thr190) was expressed in E. coli, Biotinylated. |
- Specification
- Gene Information
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Description : | Platelet-Derived Growth Factor (PDGF)-BB is synthesized as a 35 kDa, 241 amino acid (aa) prepro-precurser. It contains a signal peptide, an N-terminal prodomain, a mature region, and a C-terminal prodomain. The propercursor is initially dimerized and then intracellulary processed twice. The N-terminal prodomain is cleaved first, followed by cleavage of the C-terminal prodomain. The resulting mature region is 16-17 kDa in size (or 29-32 kDa as a homodimer). Mature human PDGF-B shares 89% aa sequence identity with mouse mature PDGF-B. PDGF-BB is expressed by hepatocytes and nonresorbing osteoclasts, generating osteoblasts and bone formation. It is also produced by platelets, macrophages, and mast cells. At sites of injury, it promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new extracellular matrix, and IGF-I-mediated re-epithelialization. The traditional receptor for PDGF is either a homodimer or heterodimer created from two type I transmembrane RTKs, PDGF R alpha and PDGF R beta. PDGF-BB has been shown to bind the alpha alpha homodimer, alpha beta heterodimer, and the beta beta homodimer in vitro, and act through the beta beta homodimer in vivo |
Source : | Mammalian cells |
Species : | Human |
Form : | Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein. |
Bio-activity : | Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5-6 ng/mL. |
Molecular Mass : | Predicted Molecular Mass: 12 kDa; SDS-PAGE: 12 kDa, reducing conditions. |
Protein length : | Ser82-Thr190 |
Endotoxin : | <1.0 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE with silver staining |
Notes : | Structure / Form: Disulfide-linked homodimer |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile |
Reconstitution : | Reconstitute at 100 μg/mL in 4 mM HCl. |
Gene Name : | PDGFB platelet-derived growth factor beta polypeptide [ Homo sapiens ] |
Official Symbol : | PDGFB |
Synonyms : | PDGFB; platelet-derived growth factor beta polypeptide; platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog) , SIS; platelet-derived growth factor subunit B; becaplermin; oncogene SIS; SSV; PDGF-2; PDGF, B chain; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor, B chain; Platelet-derived growth factor, beta polypeptide (oncogene SIS); platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); SIS; PDGF2; c-sis; FLJ12858; |
Gene ID : | 5155 |
mRNA Refseq : | NM_002608 |
Protein Refseq : | NP_002599 |
MIM : | 190040 |
UniProt ID : | P01127 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionBy potently stimulating the migratory and proliferative capabilities of smooth muscle cells and pericytes, PDGFB plays a pivotal role in regulating angiogenesis and orchestrating vascular remodeling events, thereby ensuring proper tissue perfusion and function.
PDGFB signaling assumes a critical role in fueling tumor growth, angiogenesis, and metastasis. PDGFB, acting through its activation of PDGFRβ, elicits a pro-tumorigenic cascade that promotes tumor cell proliferation, survival, migration, and invasion, while concurrently fueling tumor vascularization and distant metastatic spread.
PDGFB emerges as a significant contributor to the pathogenesis of fibrotic diseases by prompting fibroblast activation and proliferation. These dysregulated processes lead to excessive extracellular matrix deposition and subsequent tissue scarring. Furthermore, PDGFB exacerbates fibrosis through induction of myofibroblast differentiation.
Promisingly, preclinical and clinical studies have investigated diverse approaches targeting the PDGFB/PDGFRβ signaling axis for therapeutic intervention in fibrotic disorders and certain neoplastic conditions. Inhibitors directed against PDGFB or PDGFRβ have exhibited considerable potential, effectively blocking downstream signaling cascades activated by PDGFB, thereby hampering cell proliferation and angiogenesis.
PDGFB, released by platelets and macrophages at inflammatory sites, acts as a key modulator in recruiting and activating fibroblasts. This activation not only promotes collagen synthesis but also initiates tissue remodeling, thus expediting the wound healing cascade and tissue integrity restoration.
Several therapeutic strategies aim to target PDGFB for disease treatment. Inhibitors of PDGFB, such as tyrosine kinase inhibitors, have been developed and used in clinical settings. These inhibitors can selectively block the activation of PDGF receptors and inhibit downstream signaling pathways, thereby suppressing excessive cell proliferation and migration. Additionally, approaches targeting PDGFB expression, such as gene therapy or RNA interference, hold promise as potential treatments for diseases associated with PDGFB dysregulation.
PDGFB orchestrates cellular proliferative responses and drives growth by virtue of its ability to activate PDGFRβ, which in turn triggers downstream signaling cascades facilitating DNA synthesis and regulating critical cell cycle checkpoints.
Customer Reviews (3)
Write a reviewThe seal of the reagent tubes is excellent, preventing evaporation and leakage.
With its unique design and construction, the experimental reagent provides me with a fresh experimental experience.
The stability of this reagent is exceptional, ensuring the reliability of the experiment.
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