| Species : |
Human |
| Source : |
E.coli |
| Tag : |
Non |
| Protein Length : |
2-493 aa |
| Description : |
This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Form : |
Liquid |
| AASequence : |
AAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVTEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQKPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFFNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK |
| Molecular Mass : |
54-60 kDa |
| Bio-activity : |
Measured by its ability to cleave a peptide substrate, Gly-Pro-AMC. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read). The specific activity is 5013.428 pmol/min/μg. |
| Endotoxin : |
< 1 EU/μg by LAL. |
| Storage : |
Stored at -80 centigrade for 1 year from date of receipt. It is stable at -20 centigrade for 3 months after opening. It is recommended to freeze aliquots at -80 centigrade for extended storage. Avoid repeated freeze-thaw cycles. |
| Storage Buffer : |
Supplied as a 0.2 μm filtered solution of 25 mM Tris-HCl, 100 mM Glycine, 10% Glycerol, pH 8.5 or 50 mM Tris-HCL, 300 mM NaCL, 10% Glycerol, pH 7.4. |
| Shipping : |
Shipping with dry ice. |
