Human PEPD Knockdown Cell Lysate

• Cat.No.: PEPD-268HKCL
• Product Overview: WB-validated PEPD Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 55 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg PEPD KD HeLa cell lysate
• Protein Families: Druggable Genome, Protease
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: PEPD peptidase D [ Homo sapiens (human) ]
• Official Symbol: PEPD
• Synonyms: PEPD; peptidase D; xaa-Pro dipeptidase; prolidase; imidodipeptidase; X-Pro dipeptidase; proline dipeptidase; aminoacyl-L-proline hydrolase; PROLIDASE; MGC10905
• Gene ID: 5184
• mRNA Refseq: NM_000285
• Protein Refseq: NP_000276
• MIM: 613230
• UniProt ID: P12955