| Description : |
Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9A, also known as X-linked liver glycogenosis. Alternatively spliced transcript variants have been reported, but the full-length nature of these variants has not been determined. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
138.4 kDa |
| AA Sequence : |
MRSRSNSGVRLDGYARLVQQTILCY QNPVTGLLSASHEQKDAWVRDNIYS ILAVWGLGMAYRKNADRDEDKAKAY ELEQNVVKLMRGLLQCMMRQVAKVE KFKHTQSTKDSLHAKYNTATCGTVV GDDQWGHLQVDATSLFLLFLAQMTA SGLRIIFTLDEVAFIQNLVFYIEAA YKVADYGMWERGDKTNQGIPELNAS SVGMAKAALEAIDELDLFGAHGGRK SVIHVLPDEVEHCQSILFSMLPRAS TSKEIDAGLLSIISFPAFAVEDVNL VNVTKNEIISKLQGRYGCCRFLRDG YKTPREDPNRLHYDPAELKLFENIE CEWPVFWTYFIIDGVFSGDAVQVQE YREALEGILIRGKNGIRLVPELYAV PPNKVDEEYKNPHTVDRVPMGKVPH LWGQSLYILSSLLAEGFLAAGEIDP LNRRFSTSVKPDVVVQVTVLAENNH IKDLLRKHGVNVQSIADIHPIQVQP GRILSHIYAKLGRNKNMNLSGRPYR HIGVLGTSKLYVIRNQIFTFTPQFT DEHHFYLALDNEMIVEMLRIELAYL CTCWRMTGRPTLTFPISRTMLTNDG SDIHSAVLSTIRKLEDGYFGGARVK LGNLSEFLTTSFYTYLTFLDPDCDE KLFDNASEGTFSPDSDSDLVGYLED TCNQESQDELDHYINHLLQSTSLRS YLPPLCKNTEDRHVFSAIHSTRDIL SVMAKAKGLEVPFVPMTLPTKVLSA HRKSLNLVDSPQPLLEKVPESDFQW PRDDHSDVDCEKLVEQLKDCSNLQD QADILYILYVIKGPSWDTNLSGQHG VTVQNLLGELYGKAGLNQEWGLIRY ISGLLRKKVEVLAEACTDLLSHQKQ LTVGLPPEPREKIISAPLPPEELTK LIYEASGQDISIAVLTQEIVVYLAM YVRAQPSLFVEMLRLRIGLIIQVMA TELARSLNCSGEEASESLMNLSPFD MKNLLHHILSGKEFGVERSVRPIHS STSSPTISIHEVGHTGVTKTERSGI NRLRSEMKQMTRRFSADEQFFSVGQ AASSSAHSSKSARSSTPSSPTGTSS SDSGGHHIGWGERQGQWLRRRRLDG AINRVPVGFYQRVWKILQKCHGLSI DGYVLPSSTTREMTPHEIKFAVHVE SVLNRVPQPEYRQLLVEAIMVLTLL SDTEMTSIGGIIHVDQIVQMASQLF LQDQVSIGAMDTLEKDQATGICHFF YDSAPSGAYGTMTYLTRAVASYLQE LLPNSGCQMQTRTRPLEQKLISEED LAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
