Active Recombinant Human PLA2G7 Protein

• Cat.No.: PLA2G7-633H
• Product Overview: Recombinant Human PLA2G7, transcript variant 1, was expressed in HEK293 cells.

Specification

• Description: Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.
• Source: HEK293 cells
• Species: Human
• Form: Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer, 100mM NaCl, pH 7.2
• Bio-activity: Measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PAF-AH concentration of 10.0 ug/mL, 50% cleavage was achieved at an incubation time of approximately 2 minutes.
• Molecular Mass: 47 kDa
• AA Sequence: FDWQYINPVAHMKSSAWVNKIQVLM AAASFGQTKIPRGNGPYSVGCTDLM FDHTNKGTFLRLYYPSQDNDRLDTL WIPNKEYFWGLSKFLGTHWLMGNIL RLLFGSMTTPANWNSPLRPGEKYPL VVFSHGLGAFRTLYSAIGIDLASHG FIVAAVEHRDRSASATYYFKDQSAA EIGDKSWLYLRTLKQEEETHIRNEQ VRQRAKECSQALSLILDIDHGKPVK NALDLKFDMEQLKDSIDREKIAVIG HSFGGATVIQTLSEDQRFRCGIALD AWMFPLGDEVYSRIPQPLFFINSEY FQYPANIIKMKKCYSPDKERKMITI RGSVHQNFADFTFATGKIIGHMLKL KGDIDSNVAIDLSNKASLAFLQKHL GLHKDFDQWDCLIEGDDENLIPGTN INTTNQHIMLQNSSGIEKYN
• Endotoxin: Endotoxin level is <0.1 ng/µg of protein (<1EU/µg).
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: Resuspend the protein in the desired concentration in proper buffer

Gene Information

• Gene Name: PLA2G7 phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) [ Homo sapiens ]
• Official Symbol: PLA2G7
• Synonyms: PLA2G7; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma); platelet-activating factor acetylhydrolase; LDL PLA2; PAFAH; LDL-PLA(2); gVIIA-PLA2; PAF 2-acylhydrolase; PAF acetylhydrolase; group-VIIA phospholipase A2; LDL-associated phospholipase A2; lipoprotein-associated phospholipase A2; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; PAFAD; LP-PLA2; LDL-PLA2
• Gene ID: 7941
• mRNA Refseq: NM_001168357
• Protein Refseq: NP_001161829
• MIM: 601690
• UniProt ID: Q13093