Recombinant Human PLAAT3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PLAAT3-4801H
• Product Overview: PLA2G16 MS Standard C13 and N15-labeled recombinant protein (NP_009000) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: Lipid-modifying enzyme that acts as major regulator of adipocyte lipolysis by catalyzing the release of fatty acids from phospholipids in adipose tissue. Shows phospholipase A1 and A2 activity, catalyzing the calcium-independent hydrolysis of acyl groups in various phosphatidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, phospholipase A1 activity is much higher than phospholipase A2 activity. Phospholipase activity causes decreased intracellular levels of ether-type lipids, affecting peroxisome metabolism. May also have acyltransferase activity: catalyzes both N-acylation of phosphatidylethanolamine to form N-acyl-phosphatidylethanolamine and O-acylation of lyso-phosphatidylcholines to form phosphatidylcholines. The relevance of acyltransferase activity in vivo is however unclear and would require additional evidences. Also has weak lysophospholipase activity.
• Molecular Mass: 17.9 kDa
• AA Sequence: MRAPIPEPKPGDLIEIFRPFYRHWAIYVGDGYVVHLAPPSEVAGAGAASVMSALTDKAIVKKELLYDVAGSDKYQVNNKHDDKYSPLPCSKIIQRAEELVGQEVLYKLTSENCEHFVNELRYGVARSDQVRDVIIAASVAGMGLAAMSLIGVMFSRNKRQKQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PLAAT3 phospholipase A and acyltransferase 3 [ Homo sapiens (human) ]
• Official Symbol: PLAAT3
• Synonyms: PLA2G16; phospholipase A2, group XVI; HRAS like suppressor 3, HRASLS3; group XVI phospholipase A2; adipose specific PLA2; AdPLA; H REV107 1; HREV107; HREV107 3; MGC118754.; adipose-specific PLA2; HRAS-like suppressor 1; HRAS-like suppressor 3; H-rev 107 protein homolog; Ca-independent phospholipase A1/2; adipose-specific phospholipase A2; renal carcinoma antigen NY-REN-65; HRSL3; HRASLS3; HREV107-1; HREV107-3; H-REV107-1; MGC118754
• Gene ID: 11145
• mRNA Refseq: NM_007069
• Protein Refseq: NP_009000
• MIM: 613867
• UniProt ID: P53816