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Recombinant Human PLN protein, His & GST-tagged

Cat.No. : PLN-1928H
Product Overview : Recombinant Human PLN aa. (Met1~Leu52 (Accession # P26678)) fused with N-terminal His & GST tag was produced in E. coli cells.
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Description : The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.
Source : E. coli
Species : Human
Tag : His & GST
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 38.5kDa.
Protein length : Met1~Leu52 (Accession # P26678)
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 7.2.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage Buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Gene Name : PLN phospholamban [ Homo sapiens (human) ]
Official Symbol : PLN
Synonyms : Myl3; myosin, light polypeptide 3; Mylc; VLC1; MLC1s; MLC1v; MLC1SB; myosin light chain 3; myosin alkali light chain 1, ventricular/slow skeletal muscle isoform; myosin light chain 1, slow-twitch muscle B/ventricular isoform; myosin light chain, alkali, cardiac ventricles; myosin, light polypeptide 3, alkali; ventricular, skeletal, slow; slow skeletal; ventricular; ventricular/slow twitch myosin alkali light chain
Gene ID : 5350
mRNA Refseq : NM_002667.4
Protein Refseq : NP_002658.1
UniProt ID : P26678

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What is the role of PLN in the regulation of cardiac contractility? 05/11/2022

PLN regulates cardiac contractility by fine-tuning calcium cycling, influencing the force of myocardial contractions.

How does PLN contribute to the pathophysiology of heart failure? 07/03/2021

Dysregulation of PLN in heart failure results in impaired calcium handling, contributing to contractile dysfunction and the progression of heart failure.

Can you explain the structural features of PLN and its interaction with SERCA? 11/08/2020

PLN has a transmembrane domain and interacts with SERCA through its cytoplasmic domain, forming a complex that modulates calcium transport.

How does phosphorylation regulate the activity of PLN? 04/16/2019

Phosphorylation of PLN at specific sites, particularly by protein kinase A (PKA), relieves its inhibitory effect on SERCA, enhancing calcium reuptake into the sarcoplasmic reticulum.

What is the molecular function of PLN in the context of muscle contraction? 08/27/2018

PLN regulates muscle contraction by inhibiting the activity of the sarcoplasmic reticulum calcium pump (SERCA), modulating intracellular calcium levels.

Customer Reviews (3)

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Reviews
03/16/2022

    Customer service provided valuable insights into the applications of this product, helping us make an informed decision.

    03/02/2019

      The team promptly addressed my inquiries and provided valuable assistance in selecting the right recombined protein for our research.

      06/23/2018

        Lightning-fast delivery!

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