Recombinant Human POLD1 Protein, His tagged

• Cat.No.: POLD1-04H
• Product Overview: Purified recombinant protein of Human polymerase (DNA directed), delta 1, catalytic subunit 125kDa (POLD1), Arg850-end, with N-terminal His tag, expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Arg850-end
• Description: This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.
• Tag: N-His
• Molecular Mass: 29.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: > 0.05 μg/μL as determined by microplate BCA method
• Storage Buffer: 50 mM Tris-HCl, pH 8.0, 8 M urea
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.

Gene Information

• Gene Name: POLD1 DNA polymerase delta 1, catalytic subunit [ Homo sapiens (human) ]
• Official Symbol: POLD1
• Synonyms: POLD1; DNA polymerase delta 1, catalytic subunit; CDC2; MDPL; POLD; CRCS10; IMD120; DNA polymerase delta catalytic subunit; 3'-5' exodeoxyribonuclease; CDC2 homolog; DNA polymerase subunit delta p125; polymerase (DNA directed), delta 1, catalytic subunit 125kDa; polymerase (DNA) delta 1, catalytic subunit; EC 2.7.7.7
• Gene ID: 5424
• mRNA Refseq: NM_002691
• Protein Refseq: NP_002682
• MIM: 174761
• UniProt ID: P28340