Recombinant Human PPARG protein, His&GST-tagged

• Cat.No.: PPARG-7002H
• Product Overview: Recombinant Human PPARG protein(P37231-1)(Met1-Tyr505), fused with His&GST tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: Met1-Tyr505
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.4, 20% gly, 0.3mM DTTPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The recombinant human PPARG/GST chimera consists of 742 amino acids and has a calculated molecular mass of 85.4 kDa. It migrates as an approximately 85.4 kDa band in SDS-PAGE under reducing conditions as predicted.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 65 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PPARG peroxisome proliferator-activated receptor gamma [ Homo sapiens ]
• Official Symbol: PPARG
• Synonyms: PPARG; peroxisome proliferator-activated receptor gamma; peroxisome proliferative activated receptor, gamma; NR1C3; PPARG1; PPARG2; PPARgamma; PPAR gamma; PPAR-gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated nuclear receptor gamma variant 1; GLM1; CIMT1
• Gene ID: 5468
• mRNA Refseq: NM_005037
• Protein Refseq: NP_005028
• MIM: 601487
• UniProt ID: P37231