Recombinant Mouse Pparg protein, His-tagged

• Cat.No.: Pparg-39M
• Product Overview: Recombinant Mouse Pparg fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene encodes a nuclear receptor protein belonging to the peroxisome proliferator-activated receptor (Ppar) family. The encoded protein is a ligand-activated transcription factor that is involved in the regulation of adipocyte differentiation and glucose homeostasis. The encoded protein forms a heterodimer with retinoid X receptors and binds to DNA motifs termed "peroxisome proliferator response elements" to either activate or inhibit gene expression. Mice lacking the encoded protein die at an embryonic stage due to severe defects in placental vascularization. When the embryos lacking this gene are supplemented with healthy placentas, the mutants survive to term, but succumb to lipodystrophy and multiple hemorrhages. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300
• Molecular Mass: 15.6kDa
• Protein length: Phe149~Ser273
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 90%
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigradefor 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigradefor one month. Aliquot and store at -80 centigradefor 12 months.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Pparg peroxisome proliferator activated receptor gamma [ Mus musculus ]
• Official Symbol: Pparg
• Synonyms: PPARG; peroxisome proliferator activated receptor gamma; peroxisome proliferator-activated receptor gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; Nr1c3; PPARgamma; PPAR-gamma; PPARgamma2; PPAR-gamma2
• Gene ID: 19016
• mRNA Refseq: NM_001127330
• Protein Refseq: NP_001120802
• UniProt ID: P37238
• Chromosome Location: 6 53.41 cM; 6 E3-F1
• Pathway: Adipogenesis, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Gene expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem;
• Function: DNA binding; DNA binding; DNA binding; RNA polymerase II regulatory region DNA binding; activating transcription factor binding; arachidonic acid binding; chromatin binding; drug binding; enzyme binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; metal ion binding; protein binding; receptor activity; retinoid X receptor binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; steroid hormone receptor activity; transcription regulatory region DNA binding; transcription regulatory region DNA binding; zinc ion binding

Reviews

09/15/2020

It was reassuring to use a product that adhered to green chemistry principles, reducing our impact on the environment.

06/03/2017

The company's dedication to environmental sustainability reflected positively on the product and our research initiatives.

05/04/2017

We appreciated the option to request modifications or custom formulations to meet our project's requirements.

Q&As

How many isoforms of PPARG are there, and what are their weights?

PPARG has two isoforms, PPARG1 (57 kDa) and PPARG2, a splice variant with an additional 30 amino acids at the N-terminus.

What is the molecular weight of PPARG?

PPARG has two isoforms with molecular weights of 57 kDa and 54 kDa. Modified PPARG is about 67 kDa.

What does PPARG stand for?

PPARG stands for Peroxisome Proliferator-Activated Receptor Gamma.

Is there a mitochondria-localized form of PPARG?

Yes, experimental data suggests a 45 kDa protein immunologically related to PPARG, called mt-PPAR, is located in mitochondria. Its molecular weight is less than PPARG2.

What is the role of PPARG in cellular function?

PPARG is a ligand-activated transcription factor that plays a crucial role in regulating lipid homeostasis, adipogenesis, insulin resistance, and organ development.

What are the known diseases associated with PPARG defects?

Defects in PPARG are associated with familial partial lipodystrophy type 3 (FPLD3), susceptibility to obesity, type 2 insulin-resistant diabetes, hypertension, and colon cancer.

Where is PPARG mainly localized?

PPARG is mainly localized in the nucleus, although it can also be detected in the cytoplasm, exhibiting extra-nuclear/non-genomic actions in certain contexts.