Recombinant Human PRKAR1B Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PRKAR1B-4392H
• Product Overview: PRKAR1B MS Standard C13 and N15-labeled recombinant protein (NP_002726) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a regulatory subunit of cyclic AMP-dependent protein kinase A (PKA), which is involved in the signaling pathway of the second messenger cAMP. Two regulatory and two catalytic subunits form the PKA holoenzyme, disbands after cAMP binding. The holoenzyme is involved in many cellular events, including ion transport, metabolism, and transcription. Several transcript variants encoding the same protein have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 43.1 kDa
• AA Sequence: MASPPACPSEEDESLKGCELYVQLH GIQQVLKDCIVHLCISKPERPMKFL REHFEKLEKEENRQILARQKSNSQS DSHDEEVSPTPPNPVVKARRRRGGV SAEVYTEEDAVSYVRKVIPKDYKTM TALAKAISKNVLFAHLDDNERSDIF DAMFPVTHIAGETVIQQGNEGDNFY VVDQGEVDVYVNGEWVTNISEGGSF GELALIYGTPRAATVKAKTDLKLWG IDRDSYRRILMGSTLRKRKMYEEFL SKVSILESLEKWERLTVADALEPVQ FEDGEKIVVQGEPGDDFYIITEGTA SVLQRRSPNEEYVEVGRLGPSDYFG EIALLLNRPRAATVVARGPLKCVKL DRPRFERVLGPCSEILKRNIQRYNS FISLTVTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PRKAR1B protein kinase cAMP-dependent type I regulatory subunit beta [ Homo sapiens (human) ]
• Official Symbol: PRKAR1B
• Synonyms: PRKAR1B; protein kinase, cAMP-dependent, regulatory, type I, beta; cAMP-dependent protein kinase type I-beta regulatory subunit; PRKAR1
• Gene ID: 5575
• mRNA Refseq: NM_002735
• Protein Refseq: NP_002726
• MIM: 176911
• UniProt ID: P31321