"PRKN" Related Products

Recombinant Human PRKN protein

Cat.No.: PRKN-210H
Product Overview: Recombinant Human PRKN containing a substitution at Cys431Ser was expressed in Insect cells.
Description: The E3 Ubiquitin ligase Parkin (encoded by the PARK2 gene) is an essential part of the cellular machinery that participates in the removal of damaged mitochondria. Mutations in PARK2 are known to cause a form of Parkinson's disease known as autosomal recessive juvenile Parkinson's disease (AR-JP), and the mechanisms by which defective Parkin ligase contributes to the dopaminergic cell death in this disease is an area of intense investigation.
Reported substrates for Parkin include BCL2, GPR37, MIRO1, MFN1, MFN2, TOMM20, USP30, and many others. Parkin (an RBR-class Ubiquitin ligase) structures have recently been reported by multiple groups, and reveal that the ligase is folded upon itself to produce an auto-inhibited state. The auto-inhibition is relieved by interactions with PINK1 kinase (which can phosphorylate both Parkin and Ubiquitin at serine residue number 65) and pS65 phospho-Ubiquitin by mechanisms that are under investigation.

In vitro, wild-type Parkin may be activated by treatment with recombinant PINK1, or addition of low concentrations of pS65-phosphoubiquitin. Parkin has been reported to generate poly-Ubiquitin chains in K6, K11, K48, and K63 linkages both in vitro and in vivo. This recombinant protein is untagged, and is not active as determined by an autoubiquitination assay--it is intended as a negative control.
Source: Insect cells
Species: Human
Form: X mg/ml (X μM) in 25 mM Tris pH 8.5, 200 mM NaCl, 0.03% Brij 35, 10% (v/v) Glycerol, 5 mM TCEP
Bio-activity: Reaction conditions will need to be optimized for each specific application. As supplied, Parkin C431S has no E3 ligase activity as determined by the lack of autoubiquitination in an in vitro assay.
Molecular Mass: Predicted Molecular Mass: 52 kDa
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
Gene Name: PRKN parkin RBR E3 ubiquitin protein ligase [ Homo sapiens ]
Official Symbol: PRKN
Synonyms: PDJ; AR-JP; LPRS2; PARK2
Gene ID: 5071
mRNA Refseq: NM_004562
Protein Refseq: NP_004553
UniProt ID: O60260

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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