Recombinant Human PRMT5/WDR77 protein, FLAG/His-tagged

• Cat.No.: PRMT5-32H
• Product Overview: Recombinant Human PRMT5/WDR77(a.a: 2–end (both)) fused with N-terminal FLAG-tag (PRMT5) and N-terminal His-tag (WDR77) was expressed in FreeStyle 293-F cells.

Specification

• Description: Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze ADMA, while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. Type II PRMTs are found to be strongly implicated in diseases like cancer. For example, PRMT5 plays a role in the repression of certain tumor suppressor genes such as RB tumor suppressors while PRMT7 overexpression is observed in breast cancer
• Source: HEK293
• Species: Human
• Tag: FLAG/His
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 80 µg/ml Flag peptide, and 20% glycerol.
• Molecular Mass: 73 kDa (PRMT5), 37.5 kDa (MEP50)
• Purity: >75%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: At least 6 months at -80 centigrade. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: PRMT5 protein arginine methyltransferase 5 [ Homo sapiens ]
• Official Symbol: PRMT5
• Synonyms: PRMT5; protein arginine methyltransferase 5; HRMT1L5, SKB1, skb1 (S. pombe) homolog , SKB1 homolog (S. pombe); protein arginine N-methyltransferase 5; SKB1Hs; SKB1 homolog; jak-binding protein 1; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; shk1 kinase-binding protein 1 homolog; histone-arginine N-methyltransferase PRMT5; JBP1; SKB1; IBP72; HRMT1L5
• Gene ID: 10419
• mRNA Refseq: NM_006109
• Protein Refseq: NP_006100
• MIM: 604045
• UniProt ID: O14744
• Chromosome Location: 14q11.2
• Pathway: E2F transcription factor network, organism-specific biosystem; Gene expression, organism-specific biosystem; Metabolism of non-coding RNA, organism-specific biosystem; RNA transport, organism-specific biosystem; RNA transport, conserved biosystem; Signaling events mediated by HDAC Class I, organism-specific biosystem; p53 pathway, organism-specific biosystem;
• Function: histone-arginine N-methyltransferase activity; methyltransferase activity; protein binding; protein-arginine omega-N symmetric methyltransferase activity; ribonucleoprotein complex binding; transferase activity