Recombinant Human PTEN Protein, His-tagged PTEN-342H

Specification
Gene Information
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Cat.No.:	PTEN-342H
Product Overview:	Recombinant Human PTEN(Thr2~Val403) fused with His tag at N-terminal was expressed in E. coli.
Description:	This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Source:	E. coli
Species:	Human
Tag :	His
Form:	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Molecular Mass:	48.6kDa
Protein length:	Thr2~Val403
Endotoxin:	<1.0EU per 1µg (determined by the LAL method)
Purity:	> 92%
Applications:	Positive Control; Immunogen; SDS-PAGE; WB. If bio-activity of the protein is needed, please check active protein.
Stability:	The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution:	Reconstitute in PBS or others.

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Gene Name:	PTEN phosphatase and tensin homolog [ Homo sapiens ]
Official Symbol:	PTEN
Synonyms:	PTEN; phosphatase and tensin homolog; BZS, MHAM; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; MMAC1; mutated in multiple advanced cancers 1; PTEN1; TEP1; phosphatase and tensin-like protein; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; BZS; DEC; GLM2; MHAM; 10q23del; MGC11227;
Gene ID:	5728
mRNA Refseq:	NM_000314
Protein Refseq:	NP_000305
UniProt ID:	P60484