| Description : |
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His |
| Form : |
The purified protein was resolved in 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7. ) added with 300mM Imidazole and 15% glycerol. |
| Protein length : |
431-681aa |
| AA sequence : |
SHMPGDLFNLYLHQNYIDFFMEIDD IVRASEFLSFADILSGDWNTRSLLR EYSTSIATRGVMHSNKARGYAHCQG GGSSFRPLHKPQWFLINKKYRENCL AAKALFPDFCLPALCRQTQLLPYLA LLTIPMRNQAQISFIQDIGRLPLKR HFGRLKMEALTDREHGMIDPDSGDE AQLNGGHSAEESLGEPTQATVPETW SLPLSQNSASELPASQPQPFSAQGD MEENIIIEDYESDGT |
| Storage : |
Aliquot and store at -20 centigrade to -80 centigrade for up to 6 months. Avoid freeze thaw cycles. |
| Shipping : |
The product is shipped with ice packs. Upon receipt, store it immediately at -20 centigrade to -80 centigrade. |
