Recombinant Human RAD18 Protein (1-495 aa), His-SUMO-tagged

• Cat.No.: RAD18-1151H
• Product Overview: Recombinant Human RAD18 Protein (1-495 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Full Length.

Specification

• Description: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 72.2 kDa
• Protein length: 1-495 aa
• AA Sequence: MDSLAESRWPPGLAVMKTIDDLLRC GICFEYFNIAMIIPQCSHNYCSLCI RKFLSYKTQCPTCCVTVTEPDLKNN RILDELVKSLNFARNHLLQFALESP AKSPASSSSKNLAVKVYTPVASRQS LKQGSRLMDNFLIREMSGSTSELLI KENKSKFSPQKEASPAAKTKETRSV EEIAPDPSEAKRPEPPSTSTLKQVT KVDCPVCGVNIPESHINKHLDSCLS REEKKESLRSSVHKRKPLPKTVYNL LSDRDLKKKLKEHGLSIQGNKQQLI KRHQEFVHMYNAQCDALHPKSAAEI VREIENIEKTRMRLEASKLNESVMV FTKDQTEKEIDEIHSKYRKKHKSEF QLLVDQARKGYKKIAGMSQKTVTIT KEDESTEKLSSVCMGQEDNMTSVTN HFSQSKLDSPEELEPDREEDSSSCI DIQEVLSSSESDSCNSSSSDIIRDL LEEEEAWEASHKNDLQDTEISPRQN RRTRAAESAEIEPRNKRNRN
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: RAD18 RAD18 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: RAD18
• Synonyms: RAD18; RNF73; hHR18; hRAD18
• Gene ID: 56852
• mRNA Refseq: NM_020165
• Protein Refseq: NP_064550
• MIM: 605256
• UniProt ID: Q9NS91