Recombinant Human Ras Homolog Family Member G, His-tagged

• Cat.No.: RHOG-6843H
• Product Overview: Recombinant human RHOG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: RHOG is a member of the Rac subfamily of the Rho family of small G proteins. RHOG is a small monomeric GTP-binding protein, and is an important component of many intracellular signalling pathways. This protien are required for the formation of membrane ruffles during macropinocytosis. It plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 40% glycerol, 5mM DTT, 200mM NaCl.
• Molecular Mass: 25.2kDa (225aa) confirmed by MALDI-TOF
• Protein length: 1-188aa
• AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMQS IKCVVVGDGA VGKTCLLICY TTNAFPKEYI PTVFDNYSAQ SAVDGRTVNL NLWDTAGQEE YDRLRTLSYP QTNVFVICFS IASPPSYENV RHKWHPEVCH HCPDVPILLV GTKKDLRAQP DTLRRLKEQG QAPITPQQGQ ALAKQIHAVR YLECSALQQD GVKEVFAEAV RAVLNPTPIK RGRSC
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RHOG ras homolog family member G [ Homo sapiens ]
• Official Symbol: RHOG
• Synonyms: RHOG; ras homolog family member G; ARHG, ras homolog gene family, member G (rho G); rho-related GTP-binding protein RhoG; MGC125835; MGC125836; RhoG; ras homolog gene family, member G (rho G); ARHG
• Gene ID: 391
• mRNA Refseq: NM_001665
• Protein Refseq: NP_001656
• MIM: 179505
• UniProt ID: P84095
• Chromosome Location: 11p15.5-p15.4
• Pathway: Axon guidance, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Developmental Biology, organism-specific biosystem; G alpha (12/13) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPVI-mediated activation cascade, organism-specific biosystem
• Function: GTP binding; GTPase activity; nucleotide binding; protein binding