Recombinant Human RELA protein, His & T7-tagged

• Cat.No.: RELA-611H
• Product Overview: Recombinant Human RELA aa. (Pro19~Tyr306 (Accession # Q04206)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 36.6kDa
• Protein length: Pro19~Tyr306 (Accession # Q04206)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.8.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: RELA RELA proto-oncogene, NF-kB subunit [ Homo sapiens (human) ]
• Official Symbol: RELA
• Synonyms: RELA; RELA proto-oncogene, NF-kB subunit; p65; NFKB3; transcription factor p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A
• Gene ID: 5970
• mRNA Refseq: NM_001145138.1
• Protein Refseq: NP_001138610.1
• UniProt ID: Q04206