Recombinant Human RHOA

• Cat.No.: RHOA-27379TH
• Product Overview: Recombinant full length Human RhoA, MWt 22kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Ras homolog gene family, member A (RhoA) is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. In humans, it is encoded by the gene RHOA.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: pH: 7.20Constituents:0.019% Magnesium chloride, 0.595% HEPES, 0.077% DTT, 0.00088% GDP, 0.232% Sodium chloride
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the small GTPase superfamily. Rho family.
• Full Length: Full L.

Gene Information

• Gene Name: RHOA ras homolog gene family, member A [ Homo sapiens ]
• Official Symbol: RHOA
• Synonyms: RHOA; ras homolog gene family, member A; ARH12, ARHA; transforming protein RhoA; Rho12; RhoA; RHOH12
• Gene ID: 387
• mRNA Refseq: NM_001664
• Protein Refseq: NP_001655
• MIM: 165390
• Uniprot ID: P61586
• Chromosome Location: 3p21.3
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Arf6 downstream pathway, organism-specific biosystem; Aurora B signaling, organism-specific biosystem
• Function: GTP binding; GTPase activity; myosin binding; nucleotide binding; protein binding

Citation

Utility of Bioluminescent Homogeneous Nucleotide Detection Assays in Measuring Activities of Nucleotide-Sugar Dependent Glycosyltransferases and Studying Their Inhibitors

Journal: Molecules    PubMed ID: 34684811    Data: 2021/10/15

Authors: Laurie Engel, Juliano Alves, Stefan Janecek

Article Snippet:Mucin 10 (153–165) EA2 Peptide and OGT peptide substrates were purchased from AnaSpec (Fremont, CA, USA).Mucin 10 (153–165) EA2 Peptide and OGT peptide substrates were purchased from AnaSpec (Fremont, CA, USA).. Recombinant human RhoA protein was from Creative BioMart (Shirley, NY, USA).. NMX substrate was generously provided by Dr. Willie Vann (FDA).NMX substrate was generously provided by Dr. Willie Vann (FDA).

Buffers and substrates used for the Glycosyltransferase reactions.