Recombinant Human RHOT1 Protein, His-tagged

• Cat.No.: RHOT1-27H
• Product Overview: Recombinant Human RHOT1 Protein(277-618 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 277-618 aa
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• AASequence: DDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKGLFKVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR
• Purity: 80%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks). Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage. After reconstitution with sterile water, if glycerol has no effect on subsequent experiments, it is recommended to add an equal volume of glycerol for long-term storage.

Gene Information

• Gene Name: RHOT1 ras homolog family member T1 [ Homo sapiens ]
• Official Symbol: RHOT1
• Synonyms: RHOT1; ras homolog family member T1; ARHT1, ras homolog gene family, member T1; mitochondrial Rho GTPase 1; FLJ11040; MIRO 1; mitochondrial Rho (MIRO) GTPase 1; hMiro-1; mitochondrial Rho 1; ras homolog gene family, member T1; rac-GTP binding protein-like protein; rac-GTP-binding protein-like protein; ARHT1; MIRO1; MIRO-1; FLJ12633
• Gene ID: 55288
• mRNA Refseq: NM_001033566
• Protein Refseq: NP_001028738
• MIM: 613888
• UniProt ID: Q8IXI2