Recombinant Human RNF144B protein, Gst-His-tagged

• Cat.No.: RNF144B-013H
• Product Overview: Recombinant Human RNF144B fused with N-terminal GST and C-terminal His was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Description: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.
• Form: 25mM Tris,pH8.0,150mM NaCl,10% glycerol,1% Sarkosyl.
• Molecular Mass: 61.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: ring finger protein 144B [ Homo sapiens (human) ]
• Official Symbol: RNF144B
• Synonyms: PIR2; IBRDC2; p53RFP; bA528A10.3; RNF144B
• Gene ID: 255488
• mRNA Refseq: NM_182757
• Protein Refseq: NP_877434
• UniProt ID: Q7Z419