Recombinant Human SELPLG Protein, C-His-tagged

• Cat.No.: SELPLG-129H
• Product Overview: Recombinant Human SELPLG Protein with C-His tag was expressed in E. coli.

Specification

• Description: PSGL-1 (P-Selectin glycoprotein ligand, also designated CD162), exists as a disulfide-linked homodimer. PSGL-1 is a type 1 membrane protein that localizes on the tips of microvilli of leukocytes. Its extracellular domain is rich in serines, threonines and prolines, and includes a series of 15 and 16 ecameric repeats in HL-60 and U-937 cells, and human leukocytes, respectively. Although PSGL-1 appears to be the sole receptor for P-Selectin on human hematopoietic cells, it also interacts with E-Selectin through a unique binding site. In order to bind PSGL-1 to either E-Selectin or P-Selectin, PSGL-1 must be sialylated and fucosylated. PSLG-1 is a mucin-like molecule, much like leukosialin (CD43), CD164 and CD34. These proteins belong to an emerging family of cell adhesion receptors called sialomucins, which transduce negative signals in hematopoietic cells.
• Source: E. coli
• Species: Human
• Tag: C-His
• Molecular Mass: ~31 kDa
• AA Sequence: QATEYEYLDYDFLPETEPPEMLRNS TDTTPLTGPGTPESTTVEPAARRST GLDAGGAVTELTTELANMGNLSTDS AAMEIQTTQPAATEAQTTQPVPTEA QTTPLAATEAQTTRLTATEAQTTPL AATEAQTTPPAATEAQTTQPTGLEA QTTAPAAMEAQTTAPAAMEAQTTPP AAMEAQTTQTTAMEAQTTAPEATEA QTTQPTATEAQTTPLAAMEALSTEP SATEALSMEPTTKRGLFIPFSVSSV THKGIPMAASNLSVNYPVGAPDHIS VKQC
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
• Notes: For research use only, not for use in diagnostic procedure.
• Storage: Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles.
• Concentration: ≥0.5 mg/mL
• Storage Buffer: PBS, 4M Urea, pH7.4

Gene Information

• Gene Name: SELPLG selectin P ligand [ Homo sapiens (human) ]
• Official Symbol: SELPLG
• Synonyms: SELPLG; selectin P ligand; P-selectin glycoprotein ligand 1; CD162; PSGL 1; cutaneous lymphocyte-associated associated antigen; CLA; PSGL1; PSGL-1
• Gene ID: 6404
• mRNA Refseq: NM_001206609
• Protein Refseq: NP_001193538
• MIM: 600738
• UniProt ID: Q14242