Recombinant Human SEMA3A protein, His/Fc-tagged

• Cat.No.: SEMA3A-852H
• Product Overview: Recombinant Human SEMA3A(Met 1-Val 771) fused with the His tag and Fc region of human IgG1 at C-terminal was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met 1-Val 771
• Form: Lyophilized from sterile PBS, pH 7.4
• Molecular Mass: The recombinant human SEMA3A/Fc is a disulfide-linked homodimer. The reduced monomer consists of 994 amino acids and has a predicted molecular mass of 114 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh SEMA3A/Fc monomer is approximately 170-190 kDa due to glycosylation.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade~-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: SEMA3A sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A [ Homo sapiens ]
• Official Symbol: SEMA3A
• Synonyms: SEMA3A; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; SEMAD; semaphorin-3A; coll 1; Hsema I; sema III; SEMA1; SemD; collapsin 1; semaphorin D; semaphorin L; semaphorin III; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; MGC133243
• Gene ID: 10371
• mRNA Refseq: NM_006080
• Protein Refseq: NP_006071
• MIM: 603961
• UniProt ID: Q14563
• Chromosome Location: 7p12.1
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; CRMPs in Sema3A signaling, organism-specific biosystem; Developmental Biology, organism-specific biosystem; SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion, organism-specific biosystem; Sema3A PAK dependent Axon repulsion, organism-specific biosystem
• Function: chemorepellent activity; receptor activity