Recombinant Human SEMA4A protein(Met1-His683), hFc-tagged

• Cat.No.: SEMA4A-3925H
• Product Overview: Recombinant Human SEMA4A (NP_071762.2)(Met 1-His 683) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-683 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind mouse SEMA4D in a functional ELISA.
• Molecular Mass: The secreted recombinant human SEMA4A/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 893 amino acids and predictes a molecular mass of 99 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh SEMA4A/Fc monomer is approximately 110 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SEMA4A sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A [ Homo sapiens ]
• Official Symbol: SEMA4A
• Synonyms: SEMA4A; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; SEMAB; semaphorin-4A; FLJ12287; SemB; sema B; semaphorin-B; RP35; SEMB; CORD10; RP11-54H19.2
• Gene ID: 64218
• mRNA Refseq: NM_001193300
• Protein Refseq: NP_001180229
• MIM: 607292
• UniProt ID: Q9H3S1