Recombinant Human SGPL1 protein, His-tagged

• Cat.No.: SGPL1-1297H
• Product Overview: Recombinant Human SGPL1 aa. (Gln239~Arg493 fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 28kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gln239~Arg493
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.4.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SGPL1 sphingosine-1-phosphate lyase 1 [ Homo sapiens (human) ]
• Official Symbol: SGPL1
• Synonyms: SGPL1; sphingosine-1-phosphate lyase 1; SPL; S1PL; NPHS14; sphingosine-1-phosphate lyase 1; SP-lyase 1; SPL 1; hSPL; sphingosine-1-phosphate aldolase
• Gene ID: 8879
• mRNA Refseq: NM_003901.3
• Protein Refseq: NP_003892.2
• UniProt ID: O95470