Active Recombinant Human SHH Protein

• Cat.No.: SHH-6284H
• Product Overview: Active Recombinant Human SHH Protein(Q15465)(Cys24-Gly197) is expressed from E.coli without tag.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 24-197 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.1-0.4 µg/mL.
• Molecular Mass: The protein has a predicted MW of 19.79 kDa kDa. The protein migrates to 23-25 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.05 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: SHH sonic hedgehog [ Homo sapiens ]
• Official Symbol: SHH
• Synonyms: SHH; sonic hedgehog; HLP3, HPE3, sonic hedgehog (Drosophila) homolog , sonic hedgehog homolog (Drosophila); sonic hedgehog protein; HHG1; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog homolog; HLP3; HPE3
• Gene ID: 6469
• mRNA Refseq: NM_000193
• Protein Refseq: NP_000184
• MIM: 600725
• UniProt ID: Q15465