Recombinant Human SIGLEC8 protein, hFc-tagged

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Recombinant Human SIGLEC8 protein, hFc-tagged

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Cat.No. : SIGLEC8-138H
Product Overview : Recombinant Human siglec 8 Protein is expressed from mammalian with a hFc Tag at the C-terminal. It contains Met17-Ala363.
Description : Siglec-8, also known as SAF, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains. Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid.
Source : HEK293
Species : Human
Tag : hFc
Form : Supplied as 0. 22um filtered solution in PBS (pH 7. 4).
Molecular Mass : The protein has a predicted MW of 64. 6 kDa. Due to glycosylation, the protein migrates to 70-82KDa based on Bis-Tris PAGE result.
Protein length : It contains Met17-Ala363.
Endotoxin : Less than 0. 05 EU per ug by the LAL method.
Purity : > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Storage : The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Tris-Bis PAGE :

HPLC data :

Gene Name : SIGLEC8 sialic acid binding Ig-like lectin 8 [ Homo sapiens ]
Official Symbol : SIGLEC8
Synonyms : CD329 antigen; CDw329; MGC59785; SAF2; SAF2SAF-2; sialic acid binding Ig-like lectin 8; Sialoadhesin family member 2; Siglec8; Siglec-8; SIGLEC8L; SIGLEC8Lsialic acid-binding Ig-like lectin 8
Gene ID : 27181
mRNA Refseq : NM_014442
Protein Refseq : NP_055257
MIM : 605639
UniProt ID : Q9NYZ4

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