Recombinant Human SIKE1, His-tagged

• Cat.No.: SIKE1-5122H
• Product Overview: Recombinant human SIKE1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: SIKE interacts with IKK-epsilon and TBK1 and acts as a suppressor of TLR3 and virus-triggered interferon activation pathways.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
• Molecular Mass: 26.1 kDa (230aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSCTIEK ILTDAKTLLE RLREHDAAAE SLVDQSAALH RRVAAMREAG TALPDQYQED ASDMKDMSKY KPHILLSQEN TQIRDLQQEN RELWISLEEH QDALELIMSK YRKQMLQLMV AKKAVDAEPV LKAHQSHSAE IESQIDRICE MGEVMRKAVQ VDDDQFCKIQ EKLAQLELEN KELRELLSIS SESLQARKEN SMDTASQAIK
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SIKE1 suppressor of IKBKE 1 [ Homo sapiens (human) ]
• Official Symbol: SIKE1
• Synonyms: SIKE1;SIKE; RP5-1000E10.4;suppressor of IKBKE 1;suppressor of IKK epsilon;suppressor of IKK-epsilon
• Gene ID: 80143
• mRNA Refseq: NM_001102396.1
• Protein Refseq: NP_001095866.1
• MIM: 611656
• UniProt ID: Q9BRV8
• Chromosome Location: 1p13.2
• Pathway: Immune System;Innate Immune System;RIG-I-like receptor signaling pathway;RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways;TRAF3-dependent IRF activation pathway;TRAF6 mediated IRF7 activation
• Function: protein binding