"SIRPA" Related Products

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Recombinant Human SIRPA Protein, Fc-Avi-tagged, Biotinylated

Cat.No.: SIRPA-808H
Product Overview: Biotinylated Human SIRP alpha / CD172a, Fc Tag is expressed from HEK293 cells. It contains AA Glu 31 - Arg 370 (Accession # P78324-1). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.
Source: HEK293
Species: Human
Tag : Fc-Avi
Predicted N Terminal: Glu 31
Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Bio-activity: Immobilized Human CD47, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human SIRP alpha, Fc Tag with a linear range of 5-205 ug/mL.
Molecular Mass: The protein has a calculated MW of 65.8 kDa. The protein migrates as 75-105 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: SIRPA signal-regulatory protein alpha [ Homo sapiens ]
Official Symbol: SIRPA
Synonyms: SIRPA; signal-regulatory protein alpha; protein tyrosine phosphatase, non receptor type substrate 1 , PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; SIRP ALPHA 1; SIRPalpha; SIRPalpha2; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; MYD-1; CD172A; PTPNS1;
Gene ID: 140885
mRNA Refseq: NM_001040022
Protein Refseq: NP_001035111
MIM: 602461
UniProt ID: P78324

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