Recombinant Human SIRT2 Protein, His-tagged


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Recombinant Human SIRT2 Protein, His-tagged

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Cat.No. : SIRT2-23H
Product Overview : Recombinant human SIRT2(2-389) protein with a N-terminal His-tag was expressed in E. coli.
Description : This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 44.2 kDa
Protein length : 2-389
Purity : ≥90% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 1 nmol of 7-amino-4-methylcouarin per minute at 25 centigrade in 50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, containing 125 μM p53 amino acids 317-320 (Gln-Pro-Lys-Lys(e-acetyl)-AMC), and 6 mM NAD+.
Stability : ≥ 1 year
Storage : Store it at -80 centigrade
Storage Buffer : 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol
SDS-PAGE Analysis of SIRT2 :

Lane 1: MW Markers; Lane 2: SIRT2 (5 μg); Lane 3: SIRT2 (2.5 μg); Lane 4: SIRT2 (1.25 μg). Representative gel image shown; actual purity may vary between each batch.

SIRT2 Activity Assay :

Gene Name : SIRT2 sirtuin 2 [ Homo sapiens (human) ]
Official Symbol : SIRT2
Synonyms : SIRT2; sirtuin 2; SIR2; SIR2L; SIR2L2; NAD-dependent protein deacetylase sirtuin-2; NAD-dependent deacetylase sirtuin-2; SIR2-like protein 2; regulatory protein SIR2 homolog 2; silent information regulator 2; sir2-related protein type 2; sirtuin type 2
Gene ID : 22933
mRNA Refseq : NM_001193286
Protein Refseq : NP_001180215
MIM : 604480
UniProt ID : Q8IXJ6
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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