Recombinant Human SIRT2 Protein, His-tagged SIRT2-23H

Recombinant Human SIRT2 Protein, His-tagged

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Recombinant Human SIRT2 Protein, His-tagged

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Cat.No. : SIRT2-23H
Product Overview : Recombinant human SIRT2(2-389) protein with a N-terminal His-tag was expressed in E. coli.
Description : This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 44.2 kDa
Protein length : 2-389
Purity : ≥90% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 1 nmol of 7-amino-4-methylcouarin per minute at 25 centigrade in 50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, containing 125 μM p53 amino acids 317-320 (Gln-Pro-Lys-Lys(e-acetyl)-AMC), and 6 mM NAD+.
Stability : ≥ 1 year
Storage : Store it at -80 centigrade
Storage Buffer : 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol
SDS-PAGE Analysis of SIRT2 :

Lane 1: MW Markers; Lane 2: SIRT2 (5 μg); Lane 3: SIRT2 (2.5 μg); Lane 4: SIRT2 (1.25 μg). Representative gel image shown; actual purity may vary between each batch.

SIRT2 Activity Assay :

Gene Name : SIRT2 sirtuin 2 [ Homo sapiens (human) ]
Official Symbol : SIRT2
Synonyms : SIRT2; sirtuin 2; SIR2; SIR2L; SIR2L2; NAD-dependent protein deacetylase sirtuin-2; NAD-dependent deacetylase sirtuin-2; SIR2-like protein 2; regulatory protein SIR2 homolog 2; silent information regulator 2; sir2-related protein type 2; sirtuin type 2
Gene ID : 22933
mRNA Refseq : NM_001193286
Protein Refseq : NP_001180215
MIM : 604480
UniProt ID : Q8IXJ6

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