Recombinant Human Sirtuin 1, GST-tagged

• Cat.No.: SIRT1-4874H
• Product Overview: Recombinant human SIRT1 (193-end) was expressed in E. coli cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 193-end a.a.
• Description: SIRT1 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT1 being a member of class I. SIRT1 is a stress-response and chromatin-silencing factor. It is an NAD (+)-dependent histone deacetylase involved in various nuclear events such as transcription, DNA replication, and DNA repair. SIRT1 protein binds and deacetylates the p53 protein.
• Applications: Western Blot
• Molecular Weight: 105 kDa
• Expression System: E. coli
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1mM PMSF, 25 % glycerol.
• Purity: > 80 %
• Concentration: 0.2 ug/ul
• Sequences: 193-end
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: E2F transcription factor network; Energy Metabolism; FoxO family signaling; HIF-2-alpha transcription factor network; Regulation of Androgen receptor activity; Regulation of retinoblastoma protein; Signaling events mediated by HDAC Class I; Signaling events mediated by HDAC Class III

Gene Information

• Gene Name: SIRT1 sirtuin 1 [ Homo sapiens ]
• Official Symbol: SIRT1
• Synonyms: SIRT1; sirtuin 1; SIR2L1; NAD-dependent deacetylase sirtuin-1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; OTTHUMP00000019691; OTTHUMP00000198112; SIR2-like protein 1; EC 3.5.1.-; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1; NAD-dependent deacetylase sirtuin-1
• Gene ID: 23411
• mRNA Refseq: NM_001142498
• Protein Refseq: NP_001135970
• MIM: 604479
• UniProt ID: Q96EB6
• Chromosome Location: 10q21
• Function: NAD binding; NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity; deacetylase activity; histone binding; hydrolase activity; identical protein binding; metal ion binding; p53 binding; protein C-terminus binding; protein domain specific binding; transcription corepressor activity; zinc ion binding; HLH domain binding