Recombinant Human SLC12A3 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human SLC12A3 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : SLC12A3-6672H
Product Overview : SLC12A3 MS Standard C13 and N15-labeled recombinant protein (NP_000330) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 113.7 kDa
AA Sequence : MAELPTTETP GDATLCSGRF TISTLLSSDE PSPPAAYDSS HPSHLTHSST FCMRTFGYNT IDVVPTYEHY ANSTQPGEPR KVRPTLADLH SFLKQEGRHL HALAFDSRPS HEMTDGLVEG EAGTSSEKNP EEPVRFGWVK GVMIRCMLNI WGVILYLRLP WITAQAGIVL TWIIILLSVT VTSITGLSIS AISTNGKVKS GGTYFLISRS LGPELGGSIG LIFAFANAVG VAMHTVGFAE TVRDLLQEYG APIVDPINDI RIIGVVSVTV LLAISLAGME WESKAQVLFF LVIMVSFANY LVGTLIPPSE DKASKGFFSY RADIFVQNLV PDWRGPDGTF FGMFSIFFPS ATGILAGANI SGDLKDPAIA IPKGTLMAIF WTTISYLAIS ATIGSCVVRD ASGVLNDTVT PGWGACEGLA CSYGWNFTEC TQQHSCHYGL INYYQTMSMV SGFAPLITAG IFGATLSSAL ACLVSAAKVF QCLCEDQLYP LIGFFGKGYG KNKEPVRGYL LAYAIAVAFI IIAELNTIAP IISNFFLCSY ALINFSCFHA SITNSPGWRP SFQYYNKWAA LFGAIISVVI MFLLTWWAAL IAIGVVLFLL LYVIYKKPEV NWGSSVQAGS YNLALSYSVG LNEVEDHIKN YRPQCLVLTG PPNFRPALVD FVGTFTRNLS LMICGHVLIG PHKQRMPELQ LIANGHTKWL NKRKIKAFYS DVIAEDLRRG VQILMQAAGL GRMKPNILVV GFKKNWQSAH PATVEDYIGI LHDAFDFNYG VCVMRMREGL NVSKMMQAHI NPVFDPAEDG KEASARGARP SVSGALDPKA LVKEEQATTI FQSEQGKKTI DIYWLFDDGG LTLLIPYLLG RKRRWSKCKI RVFVGGQINR MDQERKAIIS LLSKFRLGFH EVHILPDINQ NPRAEHTKRF EDMIAPFRLN DGFKDEATVN EMRRDCPWKI SDEEITKNRV KSLRQVRLNE IVLDYSRDAA LIVITLPIGR KGKCPSSLYM AWLETLSQDL RPPVILIRGN QENVLTFYCQ TRTRPLEQKL ISEEDLAAND ILDYKDDDDK V
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : SLC12A3 solute carrier family 12 member 3 [ Homo sapiens (human) ]
Official Symbol : SLC12A3
Synonyms : SLC12A3; solute carrier family 12 (sodium/chloride transporters), member 3; solute carrier family 12 member 3; NCCT; Na-Cl symporter; thiazide-sensitive Na-Cl cotransporter; thiazide-sensitive sodium-chloride cotransporter; NaCl electroneutral thiazide-sensitive cotransporter; TSC; FLJ96318;
Gene ID : 6559
mRNA Refseq : NM_000339
Protein Refseq : NP_000330
MIM : 600968
UniProt ID : P55017

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