| Cat.No. : |
SMAD1-43H |
| Product Overview : |
Recombinant Human SMAD1(Met1-Ser465) fused with GST tag at N-terminal was expressed in E. coli. |
| Description : |
SMAD Family Member 1 (SMAD1) is a member of the dwarfin/SMAD family. SMAD1 has the highest expression in the heart and skeletal muscle, containing one MAD homology 1 domain and one MAD homology 2 domain, As a transcriptional modulator SMAD 1 is activated by bone morphogenetic proteins type 1 receptor kinase. Defects in SMAD1 may cause primary pulmonary hypertension (PPH1), characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure and death. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
GST |
| Form : |
Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0. |
| AA Sequence : |
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMNV TSLFSFTSPA VKRLLGWKQG DEEEKWAEKA VDALVKKLKK KKGAMEELEK ALSCPGQPSN CVTIPRSLDG RLQVSHRKGL PHVIYCRVWR WPDLQSHHEL KPLECCEFPF GSKQKEVCIN PYHYKRVESP VLPPVLVPRH SEYNPQHSLL AQFRNLGQNE PHMPLNATFP DSFQQPNSHP FPHSPNSSYP NSPGSSSSTY PHSPTSSDPG SPFQMPADTP PPAYLPPEDP MTQDGSQPMD TNMMAPPLPS EINRGDVQAV AYEEPKHWCS IVYYELNNRV GEAFHASSTS VLVDGFTDPS NNKNRFCLGL LSNVNRNSTI ENTRRHIGKG VHLYYVGGEV YAECLSDSSI FVQSRNCNYH HGFHPTTVCK IPSGCSLKIF NNQEFAQLLA QSVNHGFETV YELTKMCTIR MSFVKGWGAE YHRQDVTSTP CWIEIHLHGP LQWLDKVLTQ MGSPHNPISS VS |
| Endotoxin : |
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
| Purity : |
Greater than 95% as determined by reducing SDS-PAGE. |
| Storage : |
Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months. |
| Reconstitution : |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.