Recombinant Human SMARCA2 Protein, FLAG-tagged SMARCA2-01H

Recombinant Human SMARCA2 Protein, FLAG-tagged

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Recombinant Human SMARCA2 Protein, FLAG-tagged

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Cat.No. : SMARCA2-01H
Product Overview : Full length recombinant human SMARCA2 (BRM) Remodeling Enzyme produced in SF9 cells.
Description : The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.
Source : Insect Cells
Species : Human
Tag : FLAG
Molecular Mass : 173.6 kDa
Applications : This product is sufficient to perform 100 remodeling reactions using substrate. A single reaction is defined as 10 μl containing [20 nM SMARCA2/ 20 nM / 2.5 mM rATP] and remodels to completion in <20 minutes. 5x SMARCA Remodeling Assay Buffer is included (100 mM Tris HCl, pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20). For best results, make single-use aliquots when first thawed.
Stability : Stable for six months at -80 centigrade from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
Concentration : 0.97 mg/ml
Storage Buffer : 3.6 μl of 20 mM Tris HCl pH 7.9, 150 mM NaCl, 2 mM MgCl2, 0.2 mM EDTA, 10 mM beta-glycerophosphate, 0.01% NP-40, 0.2 mM PMSF, 0.5 mM benzamidine, 1 mM DTT, 15% glycerol, 2 μg/ml leupeptin, 1 μg/ml aprotinin, 0.4 mg/ml recombinant human insulin, 0.4 mg/ml 3x FLAG peptide. Molarity: 5.6 μM
Gene Name : SMARCA2 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 [ Homo sapiens (human) ]
Official Symbol : SMARCA2
Synonyms : SMARCA2; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a; probable global transcription activator SNF2L2; ATP-dependent helicase SMARCA2; BAF190B; BRG1-associated factor 190B; SNF2-alpha; SNF2/SWI2-like protein 2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2; brahma homolog; global transcription activator homologous sequence; protein brahma homolog; sucrose nonfermenting 2-like protein 2
Gene ID : 6595
mRNA Refseq : NM_003070
Protein Refseq : NP_003061
MIM : 600014
UniProt ID : P51531

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