Active Recombinant Human SMPD1 protein, His-tagged

• Cat.No.: SMPD1-655H
• Product Overview: Active Recombinant Human SMPD1 protein(NP_000534.3) (Gly 85-Cys 631) was expressed in Insect Cells, fused with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 85-631 aa
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol.
• Bio-activity: Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is 77 pmol/min/μg.
• Molecular Mass: The secreted recombinant human SMPD1 consists of 563 amino acids and predicts a molecular mass of 65 kDa as estimated by SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 91 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_000534.3
• Unit ID: P17405
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/P17405

Gene Information

• Gene Name: SMPD1 sphingomyelin phosphodiesterase 1, acid lysosomal [ Homo sapiens ]
• Official Symbol: SMPD1
• Synonyms: SMPD1; sphingomyelin phosphodiesterase 1, acid lysosomal; sphingomyelin phosphodiesterase; acid sphingomyelinase; ASM; NPD; ASMASE
• Gene ID: 6609
• mRNA Refseq: NM_000543
• protein Refseq: NP_000534
• MIM: 607608