Recombinant Human SPARC Protein, His-tagged

• Cat.No.: SPARC-2725H
• Product Overview: Recombinant Human SPARC Protein (Ala18-Ile303) with a N-His tag was expressed in CHO.

Specification

• Description: This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
• Source: CHO
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.3 kDa
  Accurate Molecular Mass: \
• Protein length: Ala18-Ile303
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SPARC secreted protein acidic and cysteine rich [ Homo sapiens (human) ]
• Official Symbol: SPARC
• Synonyms: SPARC; secreted protein acidic and cysteine rich; ON; ONT; OI17; BM-40; SPARC
  basement-membrane protein 40
  secreted protein, acidic, cysteine-rich (osteonectin)
• Gene ID: 6678
• mRNA Refseq: NM_003118
• Protein Refseq: NP_003109
• MIM: 182120
• UniProt ID: P09486