Recombinant Human SRY (Sex Determining Region Y)-Box 2, His-tagged

• Cat.No.: SOX2-2049H
• Product Overview: Recombinant human SOX2 protein fused to His-tag was expressed inE.coliand purified by using conventional chromatography techniques. 23.1kDa (208aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-200 a.a.
• Description: SRY (sex determining region Y)-box 2, also known as SOX2, is a transcription factor that is essential to maintain self-renewal of undifferentiated embryonic stem cells. This Protein is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 200mM NaCl, 2mM DTT, 40% glycerol.
• Purity: > 90% by SDS - PAGE.
• Concentration: 0.25 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MYNMMETELK PPGPQQTSGG GGGNSTAAAA GGNQKNSPDR VKRPMNAFMV WSRGQRRKMA QENPKMHNSE ISKRLGAEWK LLSETEKRPF IDEAKRLRAL HMKEHPDYKY RPRRKTKTLM KKDKYTLPGG LLAPGGNSMA SGVGVGAGLG AGVNQRMDSY AHMNGWSNGS YSMMQDQLGY PQHPGLNAHG AAQMQPMHRY LEHHHHHH.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: SOX2 SRY (sex determining region Y)-box 2 [ Homo sapiens ]
• Synonyms: SOX2; SRY (sex determining region Y)-box 2; ANOP3; MCOPS3; MGC2413; Transcription factor SOX-2; SRY-related HMG-box gene 2; transcription factor SOX2; sex-determining region Y-box 2
• Gene ID: 6657
• mRNA Refseq: NM_003106
• Protein Refseq: NP_003097
• MIM: 184429
• UniProt ID: P48431
• Chromosome Location: 3q26.3-q27
• Function: chromatin binding; protein binding; sequence-specific DNA binding; transcription factor activity

Citation

Structural and functional characterization of a monoclonal antibody blocking TIGIT

Journal: mAbs    PubMed ID: 35090381    Data: 2022/1/28

Authors: Bo-Seong Jeong, Hyemi Nam, Byung-Ha Oh

Article Snippet:hTIGIT-His (Creative BioMart) was used as bait to screen a na?ve human scFv phage library (Myxengo) to identify 11 clones, which were converted into IgG1-type mAbs.. The binding affinity of these mAbs for hTIGIT was analyzed by the enzyme-linked immunosorbent assay (ELISA) on a plate coated with on hTIGIT-Fc (R&D systems, Cat # 7898_TG) as previously described.The binding affinity of these mAbs for hTIGIT was analyzed by the enzyme-linked immunosorbent assay (ELISA) on a plate coated with on hTIGIT-Fc (R&D systems, Cat # 7898_TG) as previously described.