Recombinant Human STAG2 Protein, His-tagged

• Cat.No.: STAG2-2740H
• Product Overview: Recombinant Human STAG2 Protein (Met1-Leu268) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.9 kDa
  Accurate Molecular Mass: 38 kDa
• Protein length: Met1-Leu268
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: STAG2 stromal antigen 2 [ Homo sapiens (human) ]
• Official Symbol: STAG2
• Synonyms: STAG2; stromal antigen 2; cohesin subunit SA-2; SA 2; SCC3B; SCC3 homolog 2; SA2; SA-2; bA517O1.1; FLJ25871; DKFZp686P168; DKFZp781H1753
• Gene ID: 10735
• mRNA Refseq: NM_001042749
• Protein Refseq: NP_001036214
• MIM: 300826
• UniProt ID: Q8N3U4