Recombinant Human TAF12 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: TAF12-265H
• Product Overview: TAF12 MS Standard C13 and N15-labeled recombinant protein (NP_005635) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2008]
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 17.9 kDa
• AA Sequence: MNQFGPSALINLSNFSSIKPEPAST PPQGSMANSTAVVKIPGTPGAGGRL SPENNQVLTKKKLQDLVREVDPNEQ LDEDVEEMLLQIADDFIESVVTAAC QLARHRKSSTLEVKDVQLHLERQWN MWIPGFGSEEIRPYKKACTTEAHKQ RMALIRKTTKKTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TAF12 TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa [ Homo sapiens (human) ]
• Official Symbol: TAF12
• Synonyms: TAF12; TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa; TAF2J, TATA box binding protein (TBP) associated factor, RNA polymerase II, J, 20kD; transcription initiation factor TFIID subunit 12; TAFII20; TAFII20/TAFII15; TAFII-20/TAFII-15; transcription initiation factor TFIID 20/15 kDa subunits; TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD; TAF2J
• Gene ID: 6883
• mRNA Refseq: NM_005644
• Protein Refseq: NP_005635
• MIM: 600773
• UniProt ID: Q16514