|Product Overview:||Recombinant Human TIMD4(Glu25-Gln314) fused Fc region of Human IgG1 was expressed in HEK293.|
|Description:||TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain. Mature human TIM-4 consists of a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail. Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. Within the ECD human TIM-4 shares 45% aa sequence identity with mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes. It binds specifically to TIM-1 which is also the cellular receptor for hepatitis A virus and has been implicated in the development of asthma. Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells. The interaction of TIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells. TIM-4 also binds to phosphatidylserine (PS) on the surface of apoptotic cells. It enhances the clearance of apoptotic cells including T cells in the contraction phase of immune responses, apoptotic macrophages that accumulate in atherosclerosis, and dying neurons in the brain. TIM-4 is upregulated on some tumor cells and tumor-associated myeloid cells where it binds Integrin alpha V beta 3, contributes to tumor progression, and inhibits tumor-specific cellular immunity.|
|Predicted N Terminal:||Glu25|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.15-0.75 μg/mL.|
|Molecular Mass:||Predicted Molecular Mass: 58 kDa
SDS-PAGE: 95-113 kDa, reducing conditions
|Endotoxin:||<0.1 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 500 μg/mL in PBS.|
|Gene Name:||TIMD4 T-cell immunoglobulin and mucin domain containing 4 [ Homo sapiens ]|
|Synonyms:||TIMD4; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; TIM-4; TIMD-4; T-cell membrane protein 4; TIM4; SMUCKLER; FLJ27515;|
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