Recombinant Human TIMP1 Protein, His-tagged

• Cat.No.: TIMP1-132H
• Product Overview: Recombinant Human Tissue Inhibitor of Metalloproteinases 1 is produced by our Mammalian expression system and the target gene encoding Cys24-Ala207 is expressed with a 6His tag at the Cterminus.

Specification

• Description: Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13, and MMP-16. TIMP-1 does not act on MMP-14.
• Source: Human Cells
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Molecular Mass: Predicted Molecular Weight: 21.75kDa
  Apparent Molecular Weight: 27kDa, reducing conditions.
• AA Sequence: Cys24-Ala207
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >90%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: Greater than 90% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: TIMP1 TIMP metallopeptidase inhibitor 1 [ Homo sapiens (human) ]
• Official Symbol: TIMP1
• Synonyms: Metalloproteinase Inhibitor 1; Erythroid-Potentiating Activity; EPA; Fibroblast collagenase Inhibitor; Collagenase Inhibitor; Tissue Inhibitor of Metalloproteinases 1; TIMP-1; CLGI; TIMP; EPA; EPO; HCI; CLGI
• Gene ID: 7076
• mRNA Refseq: NM_003254.3
• Protein Refseq: NP_003245.1
• MIM: 305370
• UniProt ID: P01033