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Recombinant Human TIMP1 cell lysate

Cat.No. : TIMP1-2678HCL
Product Overview : Human TIMP-1 / TIMP1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : TIMP1 TIMP metallopeptidase inhibitor 1 [ Homo sapiens ]
Official Symbol : TIMP1
Synonyms : TIMP1; TIMP metallopeptidase inhibitor 1; CLGI, TIMP, tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor); metalloproteinase inhibitor 1; EPO; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1; EPA; HCI; CLGI; TIMP; FLJ90373;
Gene ID : 7076
mRNA Refseq : NM_003254
Protein Refseq : NP_003245
MIM : 305370
UniProt ID : P01033
Chromosome Location : Xp11.3-p11.23
Pathway : Activation of Matrix Metalloproteinases, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Hemostasis, organism-specific biosystem; IL6-mediated signaling events, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem;
Function : enzyme inhibitor activity; metal ion binding; metalloendopeptidase inhibitor activity; metalloendopeptidase inhibitor activity; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What are the molecular consequences of dysregulated TIMP1 expression in cancer progression? 01/06/2022

Dysregulated TIMP1 expression in cancer can disrupt the balance between MMPs and TIMPs, contributing to excessive extracellular matrix degradation, invasion, and metastasis.

Can you explain the molecular role of TIMP1 in the inhibition of metalloproteinases? 06/30/2020

TIMP1 inhibits metalloproteinases by forming a complex with them, blocking their active sites and preventing the degradation of extracellular matrix components.

How does the nuclear localization of FXN contribute to its role in cellular processes? 03/02/2020

FXN's nuclear localization is associated with its involvement in DNA repair processes, influencing genomic stability and contributing to the overall cellular response to stress.

Can you explain the interplay between TIMP1 and the immune system in inflammatory processes? 07/13/2019

TIMP1 has immunomodulatory effects, influencing inflammation by regulating immune cell migration, cytokine production, and interactions with the extracellular matrix.

What is the significance of TIMP1 in tissue homeostasis and repair? 10/19/2018

TIMP1 plays a crucial role in tissue homeostasis and repair by controlling the activity of MMPs, which are involved in remodeling the extracellular matrix during physiological and pathological processes.

Customer Reviews (3)

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Reviews
04/16/2021

    The efficient delivery of this product ensured that our research timelines were met without any complications.

    08/03/2020

      Impressed by the professionalism of the customer service team—responsive and knowledgeable about the entire product range.

      12/08/2018

        This recombined protein has become a go-to in our lab due to its reliability and the precision it brings to our experiments.

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