Active Recombinant Human TNFRSF10B Protein, His-Avi-tagged, Biotinylated

• Cat.No.: TNFRSF10B-599H
• Product Overview: Biotinylated Human TRAIL R2, His Tag is expressed from HEK293 cells. It contains AA Ile 56 - Glu 182 (Accession # O14763-1). This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Predicted N Terminal: Ile 56
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Recombinant human TNFSF10 at 5 ug/mL (100 uL/well) can bind Biotinylated Human DR5, His Tag with a linear range of 2-156 ug/mL.
• Molecular Mass: The protein has a calculated MW of 16.9 kDa. The protein migrates as 22 kDa and 24 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: TNFRSF10B tumor necrosis factor receptor superfamily, member 10b [ Homo sapiens ]
• Official Symbol: TNFRSF10B
• Synonyms: TNFRSF10B; tumor necrosis factor receptor superfamily, member 10b; tumor necrosis factor receptor superfamily member 10B; CD262; DR5; KILLER; TRAIL R2; TRICK2A; TRICKB; Fas-like protein; death receptor 5; cytotoxic TRAIL receptor-2; apoptosis inducing receptor TRAIL-R2; apoptosis inducing protein TRICK2A/2B; TNF-related apoptosis-inducing ligand receptor 2; death domain containing receptor for TRAIL/Apo-2L; tumor necrosis factor receptor-like protein ZTNFR9; p53-regulated DNA damage-inducible cell death receptor(killer); TRICK2; ZTNFR9; TRAILR2; TRICK2B; TRAIL-R2; KILLER/DR5
• Gene ID: 8795
• mRNA Refseq: NM_003842
• Protein Refseq: NP_003833
• MIM: 603612
• UniProt ID: O14763