Recombinant Human TNFRSF9 Protein, Fc & Avi-tagged, Biotinylated

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Recombinant Human TNFRSF9 Protein, Fc & Avi-tagged, Biotinylated

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Cat.No. : TNFRSF9-346H
Product Overview : Recombinant Human TNFRSF9 Protein, Fc & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # NP_001552.2). Predicted N-terminus: Leu 24. This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag. The protein has a calculated MW of 45.7 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Source : HEK293
Species : Human
Tag : Fc & Avi
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Human TNFSF9 Ligand, Fc-tag at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF9 protein, Fc & Avi-tagged, Biotinylated with a linear range of 2-19 ng/mL (QC tested).
Molecular Mass : 45.7 kDa
Protein length : Leu 24 - Gln 186
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Figure :

Gene Name : TNFRSF9 tumor necrosis factor receptor superfamily, member 9 [ Homo sapiens ]
Official Symbol : TNFRSF9
Synonyms : TNFRSF9; ILA; 4 1BB; CD137; CD137 antigen; 4-1BB; CDw137; MGC2172; FLJ43501;
Gene ID : 3604
mRNA Refseq : NM_001561
Protein Refseq : NP_001552
MIM : 602250
UniProt ID : Q07011

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