Active Recombinant Human TNFRSF18 protein, hFc-tagged, Low Endotoxin

• Cat.No.: TNFSF18-2222H
• Product Overview: Recombinant Human TNFRSF18 protein(Q9Y5U5-1)(Gln26-Glu161), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 26-161 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human GITR Ligand Trimer, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 41.3 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TNFRSF18 tumor necrosis factor receptor superfamily, member 18 [ Homo sapiens ]
• Official Symbol: TNFRSF18
• Synonyms: TNFRSF18; tumor necrosis factor receptor superfamily, member 18; tumor necrosis factor receptor superfamily member 18; AITR; CD357; GITR; activation-inducible TNFR family receptor; glucocorticoid-induced TNFR-related protein; TNF receptor superfamily activation-inducible protein; GITR-D
• Gene ID: 8784
• mRNA Refseq: NM_004195
• Protein Refseq: NP_004186
• MIM: 603905
• UniProt ID: Q9Y5U5