Recombinant Human TNNI3 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: TNNI3-095H
• Product Overview: TNNI3 MS Standard C13 and N15-labeled recombinant protein (NP_000354) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 23.8 kDa
• AA Sequence: MADGSSDAAREPRPAPAPIRRRSSN YRAYATEPHAKKKSKISASRKLQLK TLLLQIAKQELEREAEERRGEKGRA LSTRCQPLELAGLGFAELQDLCRQL HARVDKVDEERYDIEAKVTKNITEI ADLTQKIFDLRGKFKRPTLRRVRIS ADAMMQALLGARAKESLDLRAHLKQ VKKEDTEKENREVGDWRKNIDALSG MEGRKKKFESTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TNNI3 troponin I3, cardiac type [ Homo sapiens (human) ]
• Official Symbol: TNNI3
• Synonyms: TNNI3; troponin I3, cardiac type; CMD1FF; CMD2A; CMH7; cTnI; RCM1; TNNC1; troponin I, cardiac muscle; cardiomyopathy, dilated 2A (autosomal recessive); troponin I type 3 (cardiac)
• Gene ID: 7137
• mRNA Refseq: NM_000363
• Protein Refseq: NP_000354
• MIM: 191044
• UniProt ID: P19429