Active Recombinant Human TPBG protein, His/Avi-tagged, Biotinylated

• Cat.No.: TPBG-051H
• Product Overview: Biotinylated Recombinant Human TPBG(Ser31-Ser355) protein, fused to His/Avi tag at the C-terminus, was expressed in CHO cells .

Specification

• Description: TPBG, also known as Trophoblast glycoprotein and Wnt-activated inhibitory factor 1 (WAIF1), is a single-pass transmembrane protein which may function as an inhibitor
  of Wnt/beta-catenin signaling (1). Distinct regions on the 5T4 ectodomain which contains eight leucine-rich repeats are thought to antagonize Wnt signaling (2). 5T4 has
  limited expression in normal adult tissues and in some solid tumors, while expression is elevated in human carcinomas which have been correlated with poor survival
  and prognosis (3-5). Human 5T4 is a 420 amino acid (aa) protein that includes a 31 aa signal peptide, a 324 aa extracellular domain (ECD), a 21 aa transmembrane
  segment, and a 44 aa cytoplasmic tail. Within the ECD, human 5T4 shares 78% and 77% aa sequence identity with mouse and rat 5T4, respectively. It has been
  identified that TIP-2/GIPC, a common interacting protein involved in cancer, interacts with 5T4 through its PDZ domain (6). 5T4 inhibits Wnt/β-catenin signaling by
  binding to LRP6 and inhibit Wnt-induced LRP6 internalization into endocytic vesicles (1). Conversely, 5T4 can activate noncanonical Wnt signaling through DKK1 (1).
  In addition to LRP6, in-house data indicates 5T4 can also interact with Frizzled-8.
• Source: CHO cells
• Species: Human
• Tag: His/Avi
• Predicted N Terminal: Ser31
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA.
• Molecular Mass: 40-80 kDa, under reducing conditions
• Protein length: Ser31-Ser355
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 400 μg/mL in PBS.

Gene Information

• Gene Name: TPBG trophoblast glycoprotein [ Homo sapiens ]
• Official Symbol: TPBG
• Synonyms: TPBG; trophoblast glycoprotein; 5T4; 5T4 AG; 5T4 oncofetal antigen; 5T4 oncotrophoblast glycoprotein; 5T4 oncofetal trophoblast glycoprotein; M6P1; 5T4AG
• Gene ID: 7162
• mRNA Refseq: NM_001166392
• Protein Refseq: NP_001159864
• MIM: 190920
• UniProt ID: Q13641