Recombinant Human TSLP Protein, His-Avi-tagged, Biotinylated

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Recombinant Human TSLP Protein, His-Avi-tagged, Biotinylated

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Cat.No. : TSLP-1391H
Product Overview : Biotinylated Human TSLP, His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
Source : HEK293
Species : Human
Tag : His-Avi
Predicted N Terminal : Tyr 29
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity : Immobilized Human TSLP R, Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human TSLP, His Tag, Avi Tag with a linear range of 4-31 ug/mL. Immobilized Anti-TSLP, Human IgG2 at 2 ug/mL (100 uL/well) can bind Biotinylated Human TSLP, His Tag, Avi Tag with a linear range of 0.1-2 ug/mL.
Molecular Mass : The protein has a calculated MW of 18.6 kDa. As a result of glycosylation, the protein migrates as 11 kDa and 18-21 kDa under reducing condition, and 22-28 kDa under non-reducing condition (SDS-PAGE).
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >85% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : TSLP thymic stromal lymphopoietin [ Homo sapiens ]
Official Symbol : TSLP
Synonyms : TSLP; thymic stromal lymphopoietin;
Gene ID : 85480
mRNA Refseq : NM_033035
Protein Refseq : NP_149024
MIM : 607003
UniProt ID : Q969D9

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