Active Recombinant Human TSLP protein, His-Avi-tagged, Biotinylated, Low Endotoxin

• Cat.No.: TSLP-1391H
• Product Overview: Biotinylated Recombinant Human TSLP protein(Q969D9-1)(29-159 aa), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 29-159 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human TSLP, His Avi Tag at 1μg/ml(100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 17.8 kDa. Due to furin cleavage site, the protein migrates to 12 kDa and 25-30 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TSLP thymic stromal lymphopoietin [ Homo sapiens ]
• Official Symbol: TSLP
• Synonyms: TSLP; thymic stromal lymphopoietin
• Gene ID: 85480
• mRNA Refseq: NM_033035
• Protein Refseq: NP_149024
• MIM: 607003
• UniProt ID: Q969D9