Recombinant Human UB Protein, His-GFP-tagged

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Recombinant Human UB Protein, His-GFP-tagged

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Cat.No. : UB-402H
Product Overview : Recombinant Human UB Protein is produced by Bacteria expression system. This protein is fused with a His-GFP tag at the N-teminus.
Description : Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM.
Ub-GFP is a fusion protein with ubiquitin fused at the N-teminus of GFP. The protein was expressed and purified in E. Coli.
Source : Bacteria
Species : Human
Tag : His-GFP
Form : 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Molecular Mass : 35.5 kDa
Purity : > 95 % by SDS-PAGE
Storage : Store at -80 centigrade; avoid multiple freeze-thaw cycles.
Synonyms : Ubiquitin

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